BioParity.UFO / UAP research

64 papers for “Garry P. Nolan”

Results

PaperS7610

Activation of JUN in fibroblasts promotes pro-fibrotic programme and modulates protective immunity

Lu Cui, Shih-Yu Chen, Tristan Lerbs, Jin-Wook Lee, Pablo Domizi, Sydney Gordon, Yong-hun Kim, Garry Nolan, Paola Betancur, Gerlinde Wernig. ebi.ac.uk. June 3, 2020.

The authors report JUN-associated changes in lung fibroblasts and test mechanisms and interventions in engineered cultures and mouse models. Eleven end-stage cases and three cancer-resection controls define the human comparison. Preclinical findings, sampling biases and explicit toxicity cautions prevent claims of proven treatment benefit or safety in patients.

Complete supplied main text; bounded supplemental visuals
PaperS7603

Multi-omic single-cell snapshots reveal multiple independent trajectories to drug tolerance in a melanoma cell line

Yapeng Su, Melissa E Ko, Hanjun Cheng, Ronghui Zhu, Min Xue, Jessica Wang, Jihoon W Lee, Luke Frankiw, Alexander Xu, Stephanie Wong, Lidia Robert, Kaitlyn Takata, Dan Yuan, Yue Lu, Sui Huang, Antoni Ribas, Raphael Levine, Garry P Nolan, Wei Wei, Sylvia K Plevritis, Guideng Li, David Baltimore, James R Heath. ebi.ac.uk. May 11, 2020.

The authors infer different routes to early drug tolerance within one cultured melanoma cell line and test selected drug combinations. The inferred barrier was not measured. Negative inhibitor assays, dependence among cell snapshots and possible normal-tissue toxicity qualify the results; clinical efficacy was not established.

Complete supplied main text; partial supplemental visuals
PaperS7608

Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesis

Maeve O’Huallachain, Felice-Alessio Bava, Mary Shen, Carolina Dallett, Sri Paladugu, Nikolay Samusik, Simon Yu, Razika Hussein, Grantland R. Hillman, Samuel Higgins, Melanie Lou, Angelica Trejo, Laura Qin, Yu Chuan Tai, Shigemi M. Kinoshita, Astraea Jager, Deval Lashkari, Yury Goltsev, Sedide Ozturk, Garry P. Nolan. ebi.ac.uk. May 7, 2020.

The authors demonstrate split-pool barcoding for protein and targeted RNA measurements in cell lines, human blood-cell material and mouse cells. Large theoretical barcode capacity is distinct from measured event recovery. Uneven RNA detection, cell losses, exclusions, doublets and source discrepancies limit interpretation; no clinical or UAP validation follows.

Complete supplied main and correction text; partial supplemental visuals
PaperS7564

Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes

Han Chen, Christian M Schürch, Kevin Noble, Kenneth Kim, Peter O Krutzik, Erika O’Donnell, Jason Vander Tuig, Garry P Nolan, David R McIlwain. ebi.ac.uk. March 30, 2020.

A paired laboratory comparison uses three donors, three operators and eighteen processing aliquots to compare CPT and Lymphoprep methods. Differences in collection and centrifugation form part of the method packages. Nonsignificant results do not establish equivalence. Tables contain endpoint exceptions to broad conclusions, and figure captions and antibody lists conflict. Nolan contributed design, analysis and writing; commercial affiliations and funding are specifically attributed.

Complete supplied main and static supplement fields read
PaperS7626

Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza Infection

Erika Bongen, Haley Lucian, Avani Khatri, Gabriela K Fragiadakis, Zachary B Bjornson, Garry P Nolan, Paul J Utz, Purvesh Khatri. ebi.ac.uk. November 12, 2019.

The authors derive a144-gene sex-expression signature from public cohorts and explore age, inferred immune composition and influenza response. Cohort reuse and repeated observations limit independent sample counts. Associations do not establish hormone mechanisms or clinical prediction. A retraction linked inside a cited reference concerns that older paper’s epistasis interpretation; it is not a retraction of this study.

Complete supplied main, tables, meaningful figures and eight supplemental pages
PaperS7598

TRAIL-induced variation of cell signaling states provides nonheritable resistance to apoptosis

Reema Baskar, Harris G Fienberg, Zumana Khair, Patricia Favaro, Sam Kimmey, Douglas R Green, Garry P Nolan, Sylvia Plevritis, Sean C Bendall. ebi.ac.uk. November 8, 2019.

This cultured-cell study examines variation in resistance to TRAIL-induced apoptosis. Responses differ across cell lines, and no universal resistant signaling state emerged. Reviewers questioned replication, causality and selected-marker diversity; the revised paper and small surviving fractions retain those limits.

Complete supplied main text and qualified apparatus reviewed
PaperS7562

MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure

Leeat Keren, Marc Bosse, Steve Thompson, Tyler Risom, Kausalia Vijayaragavan, Erin McCaffrey, Diana Marquez, Roshan Angoshtari, Noah F. Greenwald, Harris Fienberg, Jennifer Wang, Neeraja Kambham, David Kirkwood, Garry Nolan, Thomas J. Montine, Stephen J. Galli, Robert West, Sean C. Bendall, Michael Angelo. ebi.ac.uk. October 9, 2019.

The authors describe a multiplexed tissue-imaging instrument and its performance under specific settings. The main tumor demonstration uses one patient, eight regions and 11,120 nested cells; a 41-patient comparison reuses earlier work. Static spatial patterns do not establish causal immune recruitment or clinical prediction. Nolan supported the work and held patent and shareholder interests. Sensitivity, depth counts, figure settings and panel recipes contain unresolved inconsistencies.

Complete supplied text and defined static apparatus read
PaperS7565

Role for polo-like kinase 4 in mediation of cytokinesis

Michael F Press, Bin Xie, Simon Davenport, Yu Zhou, Roberta Guzman, Garry P Nolan, Neil O’Brien, Michael Palazzolo, Tak W Mak, Joan S Brugge, Dennis J Slamon. ebi.ac.uk. May 16, 2019.

A cell-line study examines PLK4 localization and cytokinesis through antibody, genetic and inhibitor assays. The cleavage mechanism and cellular inhibitor specificity remain uncertain. Figure captions, sample panels and starting-cell counts conflict, while some replicate and animal-method details are missing. Nolan contributed reagents and analytic tools. Funding, intellectual property and commercial disclosures are specifically attributed.

Complete supplied main and static appendix read; movies unviewed
PaperS7599

Complex Mammalian-like Hematopoietic System Found in a Colonial Chordate

Benyamin Rosental, Mark Kowarsky, Jun Seita, Daniel M. Corey, Katherine J. Ishizuka, Karla J. Palmeri, Shih-Yu Chen, Rahul Sinha, Jennifer Okamoto, Gary Mantalas, Lucia Manni, Tal Raveh, D. Nathaniel Clarke, Jonathan M. Tsai, Aaron M. Newman, Norma F. Neff, Garry P. Nolan, Stephen R. Quake, Irving L. Weissman, Ayelet Voskoboynik. ebi.ac.uk. December 5, 2018.

The authors report candidate blood-forming and immune-cell populations in a colonial tunicate, using expression comparisons, transplantation and functional assays. Mammalian similarities remain qualified by missing lymphoid signatures, cross-reactivity, autofluorescence and incomplete stem-cell benchmarks.

Complete supplied manuscript and tables; apparatus missing
PaperS7589

Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis

Jared M. Churko, Priyanka Garg, Barbara Treutlein, Meenakshi Venkatasubramanian, Haodi Wu, Jaecheol Lee, Quinton N. Wessells, Shih-Yu Chen, Wen-Yi Chen, Kashish Chetal, Gary Mantalas, Norma Neff, Eric Jabart, Arun Sharma, Garry P. Nolan, Nathan Salomonis, Joseph C. Wu. ebi.ac.uk. November 21, 2018.

The study analyzes heterogeneity in cultured cardiac differentiation and tests selected regulators. Selected cells and related perturbations provide conditional support, rather than independent donor or clinical replication. A reporting-summary conflict and several negative results remain unresolved.

Supplied main text read; large workbooks partial
PaperS7571

Neurological, Cognitive, and Psychological Findings Among Survivors of Ebola Virus Disease From the 1995 Ebola Outbreak in Kikwit, Democratic Republic of Congo: A Cross-sectional Study

J Daniel Kelly, Nicole A Hoff, D’Andre Spencer, Kamy Musene, Matthew S Bramble, David McIlwain, Daniel Okitundu, Travis C Porco, George W Rutherford, M Maria Glymour, Zach Bjornson, Patrick Mukadi, Emile Okitolonda-Wemakoy, Garry P Nolan, Jean Jacques Muyembe-Tamfum, Anne W Rimoin. ebi.ac.uk. August 10, 2018.

A selected 2017 cross-sectional study compared 20 Ebola survivors with 187 contacts from the Kikwit outbreak community. Cognitive and psychological score associations do not establish persistent causal brain injury, clinical diagnoses or 22 years of longitudinal follow-up. Small samples, instrument limitations and inconsistent source values remain explicit. Nolan is a coauthor; no UAP programme is discussed.

Supplied article and supporting text read; original layouts unavailable
PaperS7614

Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging

Yury Goltsev, Nikolay Samusik, Julia Kennedy-Darling, Salil Bhate, Matthew Hale, Gustavo Vazquez, Sarah Black, Garry P. Nolan. ebi.ac.uk. August 9, 2018.

CODEX imaging maps cells and neighborhoods in nine mouse spleens. Most changing contacts track cell abundance rather than stronger inferred interactions. Neighborhood associations leave causal direction open. Image classification used test-set patches for tuning, and reported precision is not clinical accuracy. Financial interests and processing limits are retained.

Complete supplied main, figures, S1/S2 cells and S4 PDF

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