Results

PaperS7520

Highly multiplexed spatial profiling with CODEX: bioinformatic analysis and application in human disease

Wilson Kuswanto, Garry Nolan, Guolan Lu. ebi.ac.uk. November 21, 2022.

This narrative review explains CODEX tissue imaging, processing limitations and spatial analysis. Its disease examples summarize cited studies; they do not supply a new patient cohort or independent replication. The review calls for stronger causal and prospective validation.

Supplied review text and qualified figures read
PaperS7700

Postmitotic G1 phase survivin drives mitogen-independent cell division of B lymphocytes

Amit Singh, Matthew H Spitzer, Jaimy P Joy, Mary Kaileh, Xiang Qiu, Garry P Nolan, Ranjan Sen. pmc.ncbi.nlm.nih.gov. April 27, 2022.

This mouse-cell and laboratory-cell study examines continued B-cell division after removal of overt stimulation. Perturbation findings support a survivin-associated role within these models, while sufficiency and physiological generalization remain unestablished. Nolan contributed research and analytic tools; work-level public NIH/NIA support does not establish a classified or UAP approach.

Complete specialist supplied XML text; native apparatus unreviewed
PaperS7696

Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types

Gabriela K. Fragiadakis, Zachary B. Bjornson-Hooper, Deepthi Madhireddy, Karen Sachs, Han Chen, David R. McIlwain, Matthew H. Spitzer, Sean C. Bendall, Garry P. Nolan. pmc.ncbi.nlm.nih.gov. March 28, 2022.

This healthy-donor study describes correlated immune responses and sex-associated differences within one cohort. Its reported prediction tests use donors from that same cohort; clinical or causal conclusions are not established. Root reviewed results, discussion, captions and declarations, with the entire Methods section excluded. FDA contracts are acknowledged at work level without identifying Nolan as their individual recipient. Nolan advised and edited the study; these public acknowledgements do not establish a classified UAP approach.

Partial root supplied XML text; Methods excluded
PaperS7483

Subcortical Brain Morphometry Differences between Adults with Autism Spectrum Disorder and Schizophrenia

Akila Weerasekera, Adrian Ion-Mărgineanu, Garry Nolan, Maria Mody. ebi.ac.uk. March 25, 2022.

Weerasekera, Nolan and colleagues compare brain-region measurements within separate autism and schizophrenia datasets. The exploratory findings have scanner, selection and interpretation limits, including disagreements between the paper’s prose, tables and figures.

Paper text, tables and figures read
PaperS7703

A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates

Zachary B Bjornson-Hooper, Gabriela K Fragiadakis, Matthew H Spitzer, Han Chen, Deepthi Madhireddy, Kevin Hu, Kelly Lundsten, David R McIlwain, Garry P Nolan. pmc.ncbi.nlm.nih.gov. March 11, 2022.

This comparative immune atlas describes differences among sampled humans, mice and non-human primates. Mouse blood was pooled into six tubes, and the human dataset overlaps a companion study. Selected differences face antibody-affinity and unexplained denominator limits. Nolan advised the study and edited the manuscript. FDA/NIH support is acknowledged without identifying all individual award recipients; it does not establish a UAP programme or private approach. Review excludes operational biological sections, Discussion and Methods.

Partial specialist XML; operational sections and Methods excluded
PaperS7688

Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma

Darci Phillips, Magdalena Matusiak, Belén Rivero Gutierrez, Salil S Bhate, Graham L Barlow, Sizun Jiang, Janos Demeter, Kimberly S Smythe, Robert H Pierce, Steven P Fling, Nirasha Ramchurren, Martin A Cheever, Yury Goltsev, Robert B West, Michael S Khodadoust, Youn H Kim, Christian M Schürch, Garry P Nolan. ebi.ac.uk. November 18, 2021.

This observational tissue analysis links immune-cell topography to pembrolizumab response in14selected lymphoma patients from an earlier24-patient trial. Seventy regions and large cell counts remain nested within those patients. Assay validation and resampling reuse the same cohort, without an independent clinical test. Several abundance and signature measures were null; the proposed chemokine mechanism is not causally demonstrated. Nolan is a corresponding coauthor/joint supervisor. Published government grants and commercial/patent disclosures remain attributed, without establishing a classified approach or UAP programme.

Complete specialist supplied XML text; native graphics unreviewed
PaperS7685

Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging

Xavier Rovira-Clavé, Sizun Jiang, Yunhao Bai, Bokai Zhu, Graham Barlow, Salil Bhate, Ahmet F Coskun, Guojun Han, Chin-Min Kimmy Ho, Chuck Hitzman, Shih-Yu Chen, Felice-Alessio Bava, Garry P Nolan. ebi.ac.uk. July 30, 2021.

This cultured-cell imaging study maps biomolecules and drug distribution at fine spatial scales, with limited skin-tissue validation. Small cell samples and nested voxels limit generalization; 33 fields/cells across treatment groups came from one experiment. The proposed nuclear-pore pumping mechanism is an interpretation, without a demonstrated causal intervention or clinical outcome. Acknowledged NIH, Army and foundation support does not establish a secret approach or UAP programme. Nolan is a corresponding author and joint supervisor. The paper declares no competing interests; native graphics, supplements and underlying data remain unreviewed.

Complete specialist supplied XML text; native graphics unreviewed
PaperS7627

Performance of BioFire array or QuickVue influenza A + B test versus a validation qPCR assay for detection of influenza A during a volunteer A/California/2009/H1N1 challenge study

David R McIlwain, Han Chen, Maria Apkarian, Melton Affrime, Bonnie Bock, Kenneth Kim, Nilanjan Mukherjee, Garry P Nolan, Monica M McNeal. ebi.ac.uk. February 25, 2021.

The authors compare BioFire and QuickVue tests with a study-specific qPCR comparator in selected influenza-challenge samples. Repeated matched swabs come from143 volunteers, and the two comparisons use different specimen sets. A linked correction changes the rapid-test table denominator from351 to299. These results do not establish performance in community infections, current product versions or UAP research.

Complete supplied main, correction, native tables and offered static figures
PaperS7620

Nanoscopic subcellular imaging enabled by ion beam tomography

Ahmet F Coskun, Guojun Han, Shambavi Ganesh, Shih-Yu Chen, Xavier Rovira Clavé, Stefan Harmsen, Sizun Jiang, Christian M Schürch, Yunhao Bai, Chuck Hitzman, Garry P Nolan. ebi.ac.uk. February 4, 2021.

The authors demonstrate destructive ion-beam imaging and computational reconstruction in fixed cells and selected tissue. Calibration resolution, localization precision and theoretical capability remain distinct. Nested depth slices and reconstruction-derived ground truth limit independence. The method is a research demonstration, with no clinical efficacy or UAP finding established.

Complete supplied main, mathematics and figures; supplements unreceived
PaperS7617

ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs

Ivan T. Lee et al.. ebi.ac.uk. October 28, 2020.

The authors localize ACE2 staining to respiratory cilia and compare receptor expression across medication groups. Small subgroups, treatment indication, age imbalance and retrospective confounding qualify the comparisons. Negative or nonsignificant results do not establish equivalence, infection protection or clinical benefit. The study reports localization and expression rather than a treatment trial.

Complete supplied main, figures and two supplements; peer review unreviewed
PaperS7624

Coordinated Cellular Neighborhoods Orchestrate Antitumoral Immunity at the Colorectal Cancer Invasive Front

Christian M Schürch, Salil S Bhate, Graham L Barlow, Darci J Phillips, Luca Noti, Inti Zlobec, Pauline Chu, Sarah Black, Janos Demeter, David R McIlwain, Shigemi Kinoshita, Nikolay Samusik, Yury Goltsev, Garry P Nolan. ebi.ac.uk. September 3, 2020.

The authors analyze spatial immune patterns in selected colorectal-cancer tissue. Thirty-five patients supply140 dependent tissue cores; repeated cells, regions and holdouts do not create new cohorts. Survival findings and correlation-based “communication” are exploratory. A linked correction adds Shigemi Kinoshita to the author list. No clinical efficacy or UAP finding is established.

Complete supplied main, figures and correction; bounded supplementary review

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