Paper · S7560
Highly multiplexed tissue imaging using repeated oligonucleotide exchange reaction
The authors demonstrate repeated three-color imaging of DNA-tagged antibodies on five human tissue sections from five donors. Millions of segmented cells do not become independent human subjects. Barcode and repeated-cycle validation experiments each have one experimental replicate. Cell-type assignment and spatial associations depend on segmentation, markers and manual annotation. Barcode counts, omitted sequences, reagent labels, geometry and timing remain inconsistent in the supplied material. Nolan is a cosenior and corresponding coauthor.
Read the paper at ebi.ac.ukWhat we checked
All 155 supplied XML units, 39 references and 189 resource-table cells were read. All six native main figures and the graphical abstract were viewed. The complete seven-page supplement includes three figures, a 19-cycle table with 209 data positions and a second table with all 58 rows and 290 fields reviewed. Native structured publication is March 10, 2021; May issue and July PMC-release dates remain distinct. No MathML or displayed formula nodes are present. Tiny original glyphs and unprinted numeric plot values remain unknown.
Complete supplied text and received apparatus read
How we check sources
Not checked yet
- Original article-layout PDF, numbered-version and correction history, underlying data, executable code, peer review and cited full studies were not inspected. Six GIF thumbnails add no separate reading credit.
- Barcode descriptions differ between 58, 59 and up to 60. The sequence table omits B6 and validation number 17 although the panel table uses B6. A1 dye and several antibody-clone fields differ between supplied tables.
- Legends describe 31 types while density plots include 32 labels with Granulocytes.1. Peripheral-mask geometry, cycle timing, loading quantities and Hoechst names remain unresolved. Exact unprinted plot values were not recovered.
- Five selected sections and single-replicate validation constrain generalization. Spatial co-location and correlation do not prove signaling, migration, clinical validity or independent biological replication.
Source limitations
- Akoya and other industry interests, patents and funding are explicitly attributed. NIH, DoD and FDA grants do not establish a classified invitation, government programme access or UAP result.
Cite this record
Julia Kennedy‐Darling, Salil S Bhate, John W Hickey, Sarah Black, Graham L Barlow, Gustavo Vazquez, Vishal G Venkataraaman, Nikolay Samusik, Yury Goltsev, Christian M Schürch, Garry P Nolan. Highly multiplexed tissue imaging using repeated oligonucleotide exchange reaction. March 10, 2021. BioParity S7560. https://bioparity.io/sources/S7560Record data (JSON)