Results

PaperS7608

Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesis

Maeve O’Huallachain, Felice-Alessio Bava, Mary Shen, Carolina Dallett, Sri Paladugu, Nikolay Samusik, Simon Yu, Razika Hussein, Grantland R. Hillman, Samuel Higgins, Melanie Lou, Angelica Trejo, Laura Qin, Yu Chuan Tai, Shigemi M. Kinoshita, Astraea Jager, Deval Lashkari, Yury Goltsev, Sedide Ozturk, Garry P. Nolan. ebi.ac.uk. May 7, 2020.

The authors demonstrate split-pool barcoding for protein and targeted RNA measurements in cell lines, human blood-cell material and mouse cells. Large theoretical barcode capacity is distinct from measured event recovery. Uneven RNA detection, cell losses, exclusions, doublets and source discrepancies limit interpretation; no clinical or UAP validation follows.

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PaperS7571

Neurological, Cognitive, and Psychological Findings Among Survivors of Ebola Virus Disease From the 1995 Ebola Outbreak in Kikwit, Democratic Republic of Congo: A Cross-sectional Study

J Daniel Kelly, Nicole A Hoff, D’Andre Spencer, Kamy Musene, Matthew S Bramble, David McIlwain, Daniel Okitundu, Travis C Porco, George W Rutherford, M Maria Glymour, Zach Bjornson, Patrick Mukadi, Emile Okitolonda-Wemakoy, Garry P Nolan, Jean Jacques Muyembe-Tamfum, Anne W Rimoin. ebi.ac.uk. August 10, 2018.

A selected 2017 cross-sectional study compared 20 Ebola survivors with 187 contacts from the Kikwit outbreak community. Cognitive and psychological score associations do not establish persistent causal brain injury, clinical diagnoses or 22 years of longitudinal follow-up. Small samples, instrument limitations and inconsistent source values remain explicit. Nolan is a coauthor; no UAP programme is discussed.

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PaperS7577

An immune clock of human pregnancy

Nima Aghaeepour, Edward A. Ganio, David Mcilwain, Amy S. Tsai, Martha Tingle, Sofie Van Gassen, Dyani K. Gaudilliere, Quentin Baca, Leslie McNeil, Robin Okada, Mohammad S. Ghaemi, David Furman, Ronald J. Wong, Virginia D. Winn, Maurice L. Druzin, Yaser Y. El-Sayed, Cecele Quaintance, Ronald Gibbs, Gary L. Darmstadt, Gary M. Shaw, David K. Stevenson, Robert Tibshirani, Garry P. Nolan, David B. Lewis, Martin S. Angst, Brice Gaudilliere. ebi.ac.uk. September 1, 2017.

An observational immune model tracks term-pregnancy chronology in18 training and10 validation participants. Performance falls in validation; preterm pregnancies were excluded. Correlations do not establish causal immune mechanisms or preterm prediction. Source value, demographic and metadata conflicts accompany missing original article layouts and raw data. Nolan assisted interpretation and writing and disclosed equity interests.

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