{"apiVersion":"1","version":"sha256:faade27e0b75b2aca6311142ff65347b4dacc153e8d28cbdf0e8a237319331f9","data":{"id":"S7696","title":"Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types","authors":["Gabriela K. Fragiadakis","Zachary B. Bjornson-Hooper","Deepthi Madhireddy","Karen Sachs","Han Chen","David R. McIlwain","Matthew H. Spitzer","Sean C. Bendall","Garry P. Nolan"],"publishedDate":"2022-03-28","format":"Paper","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8995898/","summary":"This healthy-donor study describes correlated immune responses and sex-associated differences within one cohort. Its reported prediction tests use donors from that same cohort; clinical or causal conclusions are not established. Root reviewed results, discussion, captions and declarations, with the entire Methods section excluded. FDA contracts are acknowledged at work level without identifying Nolan as their individual recipient. Nolan advised and edited the study; these public acknowledgements do not establish a classified UAP approach.","subjects":["Research methods","Government research funding"],"people":["Garry Nolan"],"review":{"label":"Partial root supplied XML text; Methods excluded","scope":"Root directly read 46,611 of 54,480 normalized XML characters in six partition files across five untruncated returns, including five main captions, a descriptive results table, supplementary descriptions and 32 references. Entire 7,869-character Methods section excluded; full supplied text false. Native figures/PDF, supplement bodies, individual donor metadata, data, companion protocols, original contracts and correction history unreviewed.","gaps":["Entire Methods section deliberately unreviewed after the results table identified an anthrax-related stimulus. No pathogen/toxin exposure procedure or linked companion protocol was read or reproduced; full supplied-body completion is false.","Native graphics/PDF, supplementary figure and table bodies, data files, patient-level metadata and companion article/protocols were not acquired. Supplement descriptions and captions are separate from their linked files.","86 healthy-human donors appear in the body; the supplementary demographic description instead lists 39 females and 44 males (83 total). That denominator gap remains unresolved without collecting individual records. Twelve gated populations versus nine analysed cell types are separate reported scopes.","Two thousand one hundred sixty immune features were measured and 199 selected; correlated features, multiple stimuli and cell types are not independent donors. Eleven modules mostly group common signalling proteins; correlation is not demonstrated intercellular regulation or causal coordination.","Models used 50 training donors and 36 held-out donors within the same cohort. Sparse-group lasso reports 74% classification/AUC0.79; ridge68%/0.79,lasso60%/0.69,group-lasso60%/0.76. Methods, uncertainty, nesting and independent external validation were not reviewed or established here.","Seven modules and an unassigned set had stronger female correlations; module9 stronger male. Pairwise correlations share donors/features, so a very small correlation-distribution P value is not itself a causal sex effect or a clinically calibrated patient-level confidence interval.","Modules1 and7 differed after correction; other trends failed corrected significance. Eighteen features were higher in male donors and15 in female donors, while feature medians correlated0.985. These are selected-cohort associations, not universal sex categories, patient diagnosis or treatment advice.","Longitudinal stability, future disease risk, treatment response and an optimal immunization time are proposed future questions. They were not tested clinical outcomes in this healthy-donor analysis; compensatory mechanisms remain speculative.","FDA contracts HHSF223201210194C and HHSF223201610018C are acknowledged at work level, with no Nolan-specific recipient established. GF/ZB have NIH T32GM007276, GF Stanford Bio-X fellowship and MS DP5OD023056; six additional NIH awards are work support. Original contracts and award scope unverified.","Nolan advised and edited the study, not the named data-generation lead. ZB declares commercial involvement in the hosting platform; remaining authors declare no potential commercial conflicts. Article-time statements and FDA views disclaimer do not establish classified/UAP contact or programme.","The cross-species companion shares the underlying dataset; its animal counts are not new independent human cohorts. Thirty-two reference entries were read as bibliography, without following their originals. Prior accepted source scopes remain preserved.","Complete publisher correction/version history, illustrated article, all Nolan work and whole research programme remain incomplete."]},"familyId":"NOLAN-SEX-IMMUNE-SIGNALLING-2022-867016","citationUrl":"https://bioparity.io/sources/S7696","citation":"Gabriela K. Fragiadakis, Zachary B. Bjornson-Hooper, Deepthi Madhireddy, Karen Sachs, Han Chen, David R. McIlwain, Matthew H. Spitzer, Sean C. Bendall, Garry P. Nolan. Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types. March 28, 2022. BioParity S7696. https://bioparity.io/sources/S7696"}}