{"apiVersion":"1","version":"sha256:faade27e0b75b2aca6311142ff65347b4dacc153e8d28cbdf0e8a237319331f9","data":{"id":"S7683","title":"Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology","authors":["Yunhao Bai","Bokai Zhu","John-Paul Oliveria","Bryan J. Cannon","Dorien Feyaerts","Marc Bosse","Kausalia Vijayaragavan","Noah F. Greenwald","Darci Phillips","Christian M. Schürch","Samuel M. Naik","Edward A. Ganio","Brice Gaudilliere","Scott J. Rodig","Michael B. Miller","Michael Angelo","Sean C. Bendall","Xavier Rovira-Clavé","Garry P. Nolan","Sizun Jiang"],"publishedDate":"2023-07-07","format":"Paper","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10329015/","summary":"This proof-of-concept study introduces ExPRESSO for higher-resolution imaging of archived tissue on existing mass-spectrometry platforms. The reported lateral enlargement is about 3.7-fold, with axial compression, edge distortion and marker-specific signal limits. Alzheimer’s examples use six individuals, not hundreds of patients; vessel counts and image fields are nested observations. Nolan’s disclosed FDA funding and instrument-company interests are explicit. The study does not report UAP patients, a secret government approach or a clinical diagnostic trial.","subjects":["Spatial histopathology","Brain imaging","Research methods","Government research funding"],"people":["Garry Nolan"],"review":{"label":"Complete supplied XML text; original figures and supplements unreviewed","scope":"Read all 95,239 normalized supplied XML characters in ten contiguous parts, including abstract, complete 67,042-character body, six main figure captions, methods, declarations, funding metadata and 85 references. No main tables are supplied. Native figure pixels/PDF, supplementary figures and tables, peer-review file, reporting summary, source data, software and cited originals remain unreviewed.","gaps":["Complete supplied XML text and six main captions were read; native figures/PDF, supplementary figures 1–12/tables 1–10/note 1, peer-review file, reporting summary, raw imaging data, software and cited original papers remain unreviewed. Text descriptions do not constitute native pixel inspection or replication.","This is a methodological proof of concept. The expanded Alzheimer’s comparison uses six people, three with and three without clinical AD; two brain regions from one donor are not independent patients. Vessel counts 281/103, 50 fields and repeated measurements are not hundreds of participants or validated diagnostic outcomes.","Lateral expansion is approximately 3.7-fold with axial compression, not an isotropic 3D result. Edge distortion, longer scanning time for the same original area, incomplete antibody compatibility and lower signals for certain proteins are acknowledged. Claims about other molecular species and depth profiling remain prospective.","The same FDA contracts HHSF223201610018C/75F40120C00176 recur from MaxFuse; this is not a new award or secret invitation. The selected FDA metadata does not supply full agreements. Instrument-company equity/pending patent and other disclosed interests are not independently audited.","The Discussion’s workflow ordering differs from the Results/Methods description, and reference 84 carries article identifiers despite its Zenodo citation. Native layouts, underlying data and code were not checked to resolve these source-text or metadata discrepancies. No patient charts were collected or experimental protocols executed."]},"familyId":"NOLAN-EXPRESSO-EXPANDED-VACUUM-STABLE-GELS-2023","citationUrl":"https://bioparity.io/sources/S7683","citation":"Yunhao Bai, Bokai Zhu, John-Paul Oliveria, Bryan J. Cannon, Dorien Feyaerts, Marc Bosse, Kausalia Vijayaragavan, Noah F. Greenwald, Darci Phillips, Christian M. Schürch, Samuel M. Naik, Edward A. Ganio, Brice Gaudilliere, Scott J. Rodig, Michael B. Miller, Michael Angelo, Sean C. Bendall, Xavier Rovira-Clavé, Garry P. Nolan, Sizun Jiang. Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology. July 7, 2023. BioParity S7683. https://bioparity.io/sources/S7683"}}