{"apiVersion":"1","version":"sha256:faade27e0b75b2aca6311142ff65347b4dacc153e8d28cbdf0e8a237319331f9","data":{"id":"S7589","title":"Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis","authors":["Jared M. Churko","Priyanka Garg","Barbara Treutlein","Meenakshi Venkatasubramanian","Haodi Wu","Jaecheol Lee","Quinton N. Wessells","Shih-Yu Chen","Wen-Yi Chen","Kashish Chetal","Gary Mantalas","Norma Neff","Eric Jabart","Arun Sharma","Garry P. Nolan","Nathan Salomonis","Joseph C. Wu"],"publishedDate":"2018-11-21","format":"Paper","url":"https://www.ebi.ac.uk/europepmc/webservices/rest/PMC6249224/fullTextXML","summary":"The study analyzes heterogeneity in cultured cardiac differentiation and tests selected regulators. Selected cells and related perturbations provide conditional support, rather than independent donor or clinical replication. A reporting-summary conflict and several negative results remain unresolved.","subjects":["Cardiac differentiation","Single-cell research","Research limitations"],"people":["Garry Nolan"],"review":{"label":"Supplied main text read; large workbooks partial","scope":"All80,650 decoded main XML characters were read in eleven complete returns, including54 references and four captions. Root independently matched the native union and17 authors. Four main figures, six supplementary figure pages, a description page and three reporting pages were reviewed;152 antibody-table cells were read. Large workbooks have only131 prefix cells read, including seven absent positions. Four meaningful sheets in one workbook and one in the other remain partial, with next unread cellA6 on each. An empty sheet adds no meaningful completion. Original main layout and some fine plot labels remain unreviewed. November21,2018 is the native publication date.","gaps":["The initial-submission summary says all data were included and reports54 cells. Main methods describe exclusions and quality filters; results select43. Accounting remains unresolved. Lack of blinding is explicitly reported.","One adult-heart donor, ChIP-seqN=1 and related gene-edited backgrounds limit independent replication. Only one tested NR2F2 clone reproduced the comparison pattern. Cells, reads and timepoints are not additional donors or patients.","Nolan contributed CyTOF, analysis, supervision and funding. Wu disclosed a Khloris cofounder role; others declared none. Raw data, code execution, clinical benefit and UAP relevance were not established.","Original main PDF and a larger figure route failed and remain closed. Cropped gene labels, dense network details, source cross-reference conflicts and remaining workbook cells are explicit gaps."]},"familyId":"DOI-10.1038-S41467-018-07333-4","citationUrl":"https://bioparity.io/sources/S7589","citation":"Jared M. Churko, Priyanka Garg, Barbara Treutlein, Meenakshi Venkatasubramanian, Haodi Wu, Jaecheol Lee, Quinton N. Wessells, Shih-Yu Chen, Wen-Yi Chen, Kashish Chetal, Gary Mantalas, Norma Neff, Eric Jabart, Arun Sharma, Garry P. Nolan, Nathan Salomonis, Joseph C. Wu. Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. November 21, 2018. BioParity S7589. https://bioparity.io/sources/S7589"}}