{"apiVersion":"1","version":"sha256:faade27e0b75b2aca6311142ff65347b4dacc153e8d28cbdf0e8a237319331f9","data":{"id":"S7562","title":"MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure","authors":["Leeat Keren","Marc Bosse","Steve Thompson","Tyler Risom","Kausalia Vijayaragavan","Erin McCaffrey","Diana Marquez","Roshan Angoshtari","Noah F. Greenwald","Harris Fienberg","Jennifer Wang","Neeraja Kambham","David Kirkwood","Garry Nolan","Thomas J. Montine","Stephen J. Galli","Robert West","Sean C. Bendall","Michael Angelo"],"publishedDate":"2019-10-09","format":"Paper","url":"https://www.ebi.ac.uk/europepmc/webservices/rest/PMC6785247/fullTextXML","summary":"The authors describe a multiplexed tissue-imaging instrument and its performance under specific settings. The main tumor demonstration uses one patient, eight regions and 11,120 nested cells; a 41-patient comparison reuses earlier work. Static spatial patterns do not establish causal immune recruitment or clinical prediction. Nolan supported the work and held patent and shareholder interests. Sensitivity, depth counts, figure settings and panel recipes contain unresolved inconsistencies.","subjects":["Scientific background","Tissue imaging","Instrument and validation limits"],"people":["Garry Nolan"],"review":{"label":"Complete supplied text and defined static apparatus read","scope":"All 145 supplied XML units and 52 references were read, with no remaining meaningful text or tail omissions. Six main figure compositions and all seven supplement PDF pages were inspected, including the 39×39 reporter matrix, 36-channel grid and 26 distribution plots. All three published workbook rectangles were read: 1,543 cells and 1,108 nonempty cells, including 39 formula lexemes and stored caches. Root mechanically matched all cells and formula/cache records to native source values; formulas were not evaluated. Movie S1 was received but remains unviewed at its first frame/time zero. Main figures have only 272–440-pixel native extents, leaving tiny glyphs unknown.","gaps":["Movie S1 remains wholly unviewed. Original article-layout PDF, complete scientific version/correction comparison and revised date remain unknown. Six GIF thumbnails supply no extra credit. The native license states CC BY-NC 4.0.","Original Excel visual formatting, raw images, masks, repositories and analysis code were not inspected or executed. Unprinted curve coordinates and small native figure labels remain unknown; blank matrix positions are not zero-filled.","Reported indium sensitivity conflicts with its table and cerium ranking; prose and table give 15 versus 13 depths. Rounded throughput inputs differ from the stated factor. Figure pointers, fields of view, clone/isotope names and partial panel recipes remain inconsistent.","One-patient sampling, nested cells, serial-section sensitivity assumptions and reused prior cohorts constrain generalization. Static spatial relationships do not demonstrate causal recruitment, universal single-molecule sensitivity or a validated clinical predictor.","Nolan’s supporting role, instrument patent and IonPath shareholding are specifically attributed. Reported DoD funding is distinct from a government approach, classified programme or UAP finding. No private patient records or new clinical recommendation were collected."]},"familyId":"DOI-10.1126-SCIADV.AAX5851","citationUrl":"https://bioparity.io/sources/S7562","citation":"Leeat Keren, Marc Bosse, Steve Thompson, Tyler Risom, Kausalia Vijayaragavan, Erin McCaffrey, Diana Marquez, Roshan Angoshtari, Noah F. Greenwald, Harris Fienberg, Jennifer Wang, Neeraja Kambham, David Kirkwood, Garry Nolan, Thomas J. Montine, Stephen J. Galli, Robert West, Sean C. Bendall, Michael Angelo. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. October 9, 2019. BioParity S7562. https://bioparity.io/sources/S7562"}}